Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China（SCI）

2018-11-23 本站

题目：Differential distribution patterns of ammonia-oxidizing archaea and bacteria in acidic soils of Nanling National Nature Reserve forests in subtropical China（SCI）

期刊：Antonie van Leeuwenhoek

作者：Xian-Hua Gan1，Fang-Qiu Zhang2，Ji-Dong Gu3，Yue-Dong Guo1，Zhao-Qing Li1，Wei-Qiang Zhang1，Xiu-Yu Xu1，Yi Zhou1，Xiao-Ying Wen1，Guo-Guang Xie4，Yong-Feng Wang1 2，

单位：1.Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and PestGuangdong Academy of ForestryGuangzhouPeople’s Republic of China；2.Laboratory of Microbial Ecology and ToxicologyGuangdong Academy of ForestryGuangzhouPeople’s Republic of China； 3.Laboratory of Environmental Microbiology and Toxicology, School of Biological SciencesThe University of Hong KongHong Kong SARPeople’s Republic of China； 4.Nanling National Nature ReserveShaoguanPeople’s Republic of China

摘要： In addition to ammonia-oxidizing bacteria (AOB) the more recently discovered ammonia-oxidizing archaea (AOA) can also oxidize ammonia, but little is known about AOA community structure and abundance in subtropical forest soils. In this study, both AOA and AOB were investigated with molecular techniques in eight types of forests at surface soils (0–2 cm) and deep layers (18–20 cm) in Nanling National Nature Reserve in subtropical China. The results showed that the forest soils, all acidic (pH 4.24–5.10), harbored a wide range of AOA phylotypes, including the genera Nitrosotalea, Nitrososphaera, and another 6 clusters, one of which was reported for the first time. For AOB, only members of Nitrosospira were retrieved. Moreover, the abundance of the ammonia monooxygenase gene (amoA) from AOA dominated over AOB in most soil samples (13/16). Soil depth, rather than forest type, was an important factor shaping the community structure of AOA and AOB. The distribution patterns of AOA and AOB in soil layers were reversed: AOA diversity and abundances in the deep layers were higher than those in the surface layers; on the contrary, AOB diversity and abundances in the deep layers were lower than those in the surface layers. Interestingly, the diversity of AOA was positively correlated with pH, but negatively correlated with organic carbon, total nitrogen and total phosphorus, and the abundance of AOA was negatively correlated with available phosphorus. Our results demonstrated that AOA and AOB were differentially distributed in acidic soils in subtropical forests and affected differently by soil characteristics.

关键词：Ammonia-oxidizing archaea ，Subtropical forest ，Acidic soil，Thaumarchaeota Nanling Mountains