Characterization of a collection of Chinese fir elite genotypes using sequence-related amplified polymorphism markers

2018-11-23 本站

题目：Characterization of a collection of Chinese fir elite genotypes using sequence-related amplified polymorphism markers

期刊：Journal of Forestry Research

作者：Hongjing Duan1,2，Dehuo Hu2，Yun Li1，Huiquan Zheng2

单位：1.National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education, College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingPeople’s Republic of China；2.Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and PestGuangdong Academy of ForestryGuangzhouPeople’s Republic of China

摘要： Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a major timber tree species in China. In the present study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the polymorphisms, diversity, and relationships in a collection of Chinese fir elite genotypes (n = 103) from the Lechang provenance, an endangered provenance. Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes; 577 (93.1 %) of these were polymorphic, yielding an average of 16.5 polymorphic bands per primer combination. Of the SRAP primer combinations (n = 35), 14 ones identified ≥70.0 % of the 120 genotypes. The Me12–Em19 combination had the strongest discriminatory ability with 93.3 % of the genotypes identified. An UPGMA dendrogram further showed that most of the Lechang provenance genotypes (87.4 %) grouped together (Cluster A). The Lechang genotypes had a considerable amount of 美高梅 variation with 美高梅 similarity from 0.40 to 0.81. The current work may facilitate the management of the endangered Lechang Chinese fir provenance.

关键词：Chinese fir，SRAP Germplasm，Genetic variation，Dendrogram